abstract：:Tetrahydrobiopterin (BH4) is a ubiquitous pteridine metabolite that serves as a NOS cofactor. Recently, we showed that BH4 efficiently inhibits superoxide generation from the heme group at the oxygenase domain of eNOS. This role indicates that BH4 acts as a redox switch in the catalytic mechanism of the enzyme, which ...

journal_title：Free radical research

authors： Vásquez-Vivar J,Kalyanaraman B,Martásek P

更新日期：2003-02-01 00:00:00

abstract：:In the present study we tested the ability of different antioxidant agents, used alone or in combination, to reduce the reactive oxygen species (ROS) levels and to increase the glutathione peroxidase (GPx) activity. Moreover, we tested the ability of such antioxidant agents to reduce the serum levels of proinflammator...

journal_title：Free radical research

authors： Mantovani G,Macciò A,Madeddu C,Mura L,Gramignano G,Lusso MR,Murgia V,Camboni P,Ferreli L,Mocci M,Massa E

更新日期：2003-02-01 00:00:00

abstract：:α-Tocopherol (a form of vitamin E) is a fat-soluble vitamin that can prevent lipid peroxidation of cell membranes. This antioxidant activity of α-tocopherol can help to prevent cardiovascular disease, atherosclerosis and cancer. We investigated the α-tocopherol level and the expression of α-tocopherol transfer protein...

journal_title：Free radical research

authors： Misaki K,Takitani K,Ogihara T,Inoue A,Kawakami C,Kuno T,Kawamura N,Miyake M,Nakagawa T,Tamai H

更新日期：2003-01-01 00:00:00

abstract：:The aim of this study was to investigate the effect of HDL oxidation on PON1 paraoxonase activity. Also, we were interested in investigating the mechanism by which PON1 could be inactivated and the correlation between its enzymatic activity and the antioxidant properties of HDL. Three different oxidation systems were ...

journal_title：Free radical research

authors： Jaouad L,Milochevitch C,Khalil A

更新日期：2003-01-01 00:00:00

abstract：:Aging is a multifold process affected by many genes and thus many biochemical pathways. This conclusion is underscored by the failure to find simple central controls for the aging process during the 20th Century. This situation poses a fundamental challenge to anti-aging medicine: how to develop effective therapies fo...

journal_title：Free radical research

authors： Rose MR,Mueller LD,Long AD

更新日期：2002-12-01 00:00:00

abstract：:Several substances related to the neurodegenerative diseases of Alzheimer and Parkinson, such as hydrogen peroxide, tumor necrosis factor alpha, dopamine and beta-amyloid peptide 1-42, have been shown to induce apoptosis in tumoral cell lines and rat neurons but not in human neurons. Moreover, the role of mitochondria...

journal_title：Free radical research

authors： Medina S,Martínez M,Hernanz A

更新日期：2002-11-01 00:00:00

abstract：:The host inflammatory response appears to be an important contributor to the pathogenesis of human viral respiratory illness. Virus-induced oxidative stress appears to mediate an early phase of elaboration of the proinflammatory cytokine interleukin-8 by respiratory epithelial cells. The purpose of these studies was t...

journal_title：Free radical research

authors： Kaul P,Singh I,Turner RB

更新日期：2002-10-01 00:00:00

abstract：:During the early postimplantation period, rodent embryos survive in a relatively anaerobic environment in utero and are vulnerable to a high oxygen pressure. They become resistant to oxygen stress when they are exposed to a higher oxygen pressure after the uteroplacental circulation is established. However, it is unkn...

journal_title：Free radical research

authors： Kobayashi-Miura M,Nakamura H,Yodoi J,Shiota K

更新日期：2002-09-01 00:00:00

abstract：:2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC) has been identified as a major water-soluble metabolite of vitamin E, which circulates in the blood and is excreted with the urine. The aim of this study was to assess the antioxidant activity of alpha-CEHC using several methods with different proox...

journal_title：Free radical research

authors： Betancor-Fernandez A,Sies H,Stahl W,Polidori MC

更新日期：2002-08-01 00:00:00

abstract：:A glycomic approach to the identification of target molecules in glycosyltransferase gene targeting mice is a promising strategy to understand the biological significance of glycosyltransferase genes in vivo. In order to understand the biological effects of N-acetylglucosaminyltransferase III (GnT-III) on tumor format...

journal_title：Free radical research

authors： Ekuni A,Miyoshi E,Ko JH,Noda K,Kitada T,Ihara S,Endo T,Hino A,Honke K,Taniguchi N

更新日期：2002-08-01 00:00:00

abstract：:Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human p...

journal_title：Free radical research

authors： Müller K,Carpenter KL,Challis IR,Skepper JN,Arends MJ

更新日期：2002-07-01 00:00:00

abstract：:The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up in 1997 to resolve methodological problems and to reach agreement on the basal level of 8-oxo-2'-deoxyguanosine (8-oxodG) in biological samples. In the present ESCODD trial 6 samples of 8-oxodG-containing oligonucleotides with different ratio...

journal_title：Free radical research

authors： Riis B

更新日期：2002-06-01 00:00:00

abstract：:Glucose at pathophysiological concentrations was able to accelerate copper-induced oxidation of isolated low-density lipoprotein (LDL) and whole serum. The efficiency of glucose was favored under the following circumstances: (a) when LDL oxidation was induced by low copper concentration, (b) when LDL was partly oxidiz...

journal_title：Free radical research

authors： Leoni V,Albertini R,Passi A,Abuja PM,Borroni P,D'Eril GM,De Luca G

更新日期：2002-05-01 00:00:00

abstract：:The oxidative stress possibly resulting from an inherited respiratory chain (RC) deficiency was investigated in a series of human cultured skin fibroblasts presenting either ubiquinone depletion or isolated defect of the various RC complexes. Taken as an index for superoxide overproduction, a significant induction of ...

journal_title：Free radical research

authors： Geromel V,Rötig A,Munnich A,Rustin P

更新日期：2002-04-01 00:00:00

abstract：:Coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport gene as well as an important antioxidant, which is particularly effective within mitochondria. A number of prior studies have shown that it can exert efficacy in treating patients with known mitochondrial disorders. We investigated the potential u...

journal_title：Free radical research

authors： Beal MF

更新日期：2002-04-01 00:00:00

abstract：:Degenerative diseases such as cancer are induced by oxidative genetic damage. Antioxidants can scavenge reactive oxygen species, but to prevent disease, they must do so quickly, before the DNA bases are damaged. In the present study, a novel method was established for evaluating the potency of antioxidants employing 2...

journal_title：Free radical research

authors： Sakakibara H,Ashida H,Kanazawa K

更新日期：2002-03-01 00:00:00

abstract：:Oxidized low density lipoprotein (LDL) plays an important role in atherogenesis. It is generally thought that LDL is mainly oxidized in the intima of vessel walls, surrounded by hydrophilic antioxidants and proteins such as albumin. The aim of this study was to investigate the possible interrelationships between oxida...

journal_title：Free radical research

authors： Carbonneau MA,Cartron E,Leger CL,Senglat C,Descomps B

更新日期：2002-02-01 00:00:00

abstract：:Carotenoids scavenge simple lipid-like alkylperoxyl radicals. However, the rate constant is too low to be determined directly and the mechanism is likewise not known with certainty [Mortensen, A. and Skibsted, L.H. (1998) FEBS Lett. 426, 392-396]. It is demonstrated that carotenoids react with peroxyl radicals only sl...

journal_title：Free radical research

authors： Mortensen A

更新日期：2002-02-01 00:00:00

abstract：:The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests...

journal_title：Free radical research

authors： Kirschvink N,Fiévez L,Bureau F,Degand G,Maghuin-Rogister G,Smith N,Art T,Lekeux P

更新日期：2002-01-01 00:00:00

abstract：:The in vitro toxicity of the reactive oxygen species generating enzyme xanthine oxidoreductase (XOR) to human peripheral blood lymphocytes was studied after stimulation with phytohaemoagglutinin or anti-CD3/CD28 antibodies. Apoptosis and necrosis were induced by the XOR/hypoxanthine system in a time- and concentration...

journal_title：Free radical research

authors： Battelli MG,Musiani S,Tazzari PL,Stirpe F

更新日期：2001-12-01 00:00:00

abstract：:We investigated the effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, on reactive oxygen species (ROS) and on oxidative DNA damage in vitro, as well as the effects of the main fluvastatin metabolites (M2, M3, and M4) and other inhibitors of the same enzyme, pravastatin and simvastati...

journal_title：Free radical research

authors： Imaeda A,Tanigawa T,Aoki T,Kondo Y,Nakamura N,Yoshikawa T

更新日期：2001-12-01 00:00:00

abstract：:The antioxidative activity of hydroxylamines was evaluated for the oxidation of tetralin at 61 degrees C and linoleic acid micelles in an aqueous dispersion at 37 degrees C, induced by an azo initiator. The antioxidative efficacy of the hydroxylamines for the oxidation of tetralin was smaller than that of alpha-tocoph...

journal_title：Free radical research

authors： Nishiyama T,Tamekuni H,Tachibana T,Kitamura H,Saito S

更新日期：2001-12-01 00:00:00

abstract：:To examine the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) in the oxidative stress-induced increase of permeability in endothelial cells, the effects of a p38 MAPK inhibitor (SB203580) and ERK inhibitor (PD90859) on the H2O2-induced increase of permeab...

journal_title：Free radical research

authors： Niwa K,Inanami O,Ohta T,Ito S,Karino T,Kuwabara M

更新日期：2001-11-01 00:00:00

abstract：:Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermore, antipyrine react...

journal_title：Free radical research

authors： Meijer EP,Coolen SA,Bast A,Westerterp KR

更新日期：2001-10-01 00:00:00

abstract：:To determine effect of nitric oxide (NO) on cellular glutathione peroxidase (GPX) level in living cells, we measured the activity, protein and mRNA of GPX in rat kidney (KNRK) cells under a high NO condition. Combined treatment of lipopolysaccharide (LPS, 1 microgram/ml) and tumor necrosis factor-alpha (TNF-alpha, 50 ...

journal_title：Free radical research

authors： Dobashi K,Asayama K,Nakane T,Kodera K,Hayashibe H,Nakazawa S

更新日期：2001-09-01 00:00:00

abstract：:Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the end...

journal_title：Free radical research

authors： Giakoustidis D,Kontos N,Iliadis S,Papageorgiou G,Tsantilas D,Spyridis C,Papazoglou K,Botsoglou N,Dimitriadou A,Giakoustidis E

更新日期：2001-08-01 00:00:00

abstract：:The purpose of this study was to investigate the absorption and metabolism of hydroxycinnamates from artichoke extract by determining the urinary excretion of the conjugates. Ten healthy, non smoking volunteers (5 female, 5 male) were given three capsules containing artichoke extract every 4 h (0, 4, 8 h) following tw...

journal_title：Free radical research

authors： Rechner AR,Pannala AS,Rice-Evans CA

更新日期：2001-08-01 00:00:00

abstract：:Radicals from one-electron oxidation of 4-hydroxycinnamate, ferulate and 3,4-dihydroxycinnamate have been formed by reaction with the oxidising triplet state of duroquinone. All three compounds react with triplet duroquinone with second order rate constants close to the diffusion-controlled limit. The identity of the ...

journal_title：Free radical research

authors： Bisby RH,Parker AW

更新日期：2001-07-01 00:00:00

abstract：:We have reported previously that the apparent rate of peroxynitrite (ONOO(-) ) decay, as followed from its absorbance at 302 nm, decreases in the presence of hydrogen peroxide, mannitol and ethanol (Alvarez et al., 1995, Chem. Res. Toxicol. 8:859-864; Alvarez et al., 1998, Free Radic. Biol. Med. 24:1331-1337). Recentl...

journal_title：Free radical research

authors： Alvarez B,Radi R

更新日期：2001-05-01 00:00:00

abstract：:Manganese superoxide dismutase (MnSOD) is essential for life as dramatically illustrated by the neonatal lethality of mice that are deficient in MnSOD. In addition, mice expressing only 50% of the normal compliment of MnSOD demonstrate increased susceptibility to oxidative stress and severe mitochondrial dysfunction r...

journal_title：Free radical research

authors： Macmillan-Crow LA,Cruthirds DL

更新日期：2001-04-01 00:00:00

abstract：:Involvement of oxidative stress is implicated in the progression of complication of diabetes mellitus. With respect to heart diseases, we have studied role of oxidative stress/antioxidants using rats treated with streptozotocin to induce diabetes (DM). Hemodynamic and echocardiographic measurements showed thickening o...

journal_title：Free radical research

authors： Doi K,Sawada F,Toda G,Yamachika S,Seto S,Urata Y,Ihara Y,Sakata N,Taniguchi N,Kondo T,Yano K

更新日期：2001-03-01 00:00:00

abstract：:Neopterin and the reduced form, 7,8-dihydroneopterin (78NP) are pteridines released from macrophages when stimulated with gamma-interferon in vivo. The role of 78NP in inflammatory response is unknown though neopterin has been used clinically as a marker of immune cell activation, due to its very fluorescent nature. U...

journal_title：Free radical research

authors： Gieseg SP,Maghzal G,Glubb D

更新日期：2001-02-01 00:00:00

abstract：:Nine male volunteers were given 36 g of cocoa powder (containing 2610 mg of polyphenols) per day with sugar and 6 volunteers received an equivalent amount of sugar for 2 weeks. Conjugated diene production in LDL induced by 2-2' azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) and copper ion were evaluated. The lag t...

journal_title：Free radical research

authors： Osakabe N,Baba S,Yasuda A,Iwamoto T,Kamiyama M,Takizawa T,Itakura H,Kondo K

更新日期：2001-01-01 00:00:00

abstract：:Gastric inflammation is a highly complex biochemical protective response to cellular/tissue injury. When this process occurs in an uncontrolled manner, the result is excessive cellular/tissue damage that results chronic inflammation and destruction of normal tissue. Current evidence suggests that Helicobacter pylori (...

journal_title：Free radical research

authors： Yoshikawa T,Naito Y

更新日期：2000-12-01 00:00:00

abstract：:Validation of markers requires two different steps. The first one is the analytical validation including analytical procedures, definition of standard reference, and round-tests analysis. The second one is the assessment of the biological relevance of the selected marker. This biological relevance may be in the field ...

journal_title：Free radical research

authors： Antoine JM,Diplock AT

更新日期：2000-11-01 00:00:00

abstract：:Proteins constitute the major 'working force' for all forms of biological work. Their exact conformation and pattern of folding are tightly connected to their activity and function. Reactive oxygen and nitrogen species (ROS and RNS) are formed during normal metabolism and in higher fluxes under pathological conditions...

journal_title：Free radical research

authors： Chevion M,Berenshtein E,Stadtman ER

更新日期：2000-11-01 00:00:00

abstract：:Increased reactive oxygen species (ROS) and lipid peroxidation may be implicated in the pathogenesis of preeclampsia by causing cell (membrane) damage and impaired endothelial function. Carbonyl derivatives of proteins, or protein carbonyls, may be sensitive biomarkers of ROS-mediated damage. The aim of the study was ...

journal_title：Free radical research

authors： Zusterzeel PL,Mulder TP,Peters WH,Wiseman SA,Steegers EA

更新日期：2000-11-01 00:00:00

abstract：:Oxidized low density lipoprotein (LDL) has a major impact in the development of atherosclerosis. Risk for oxidative modification of LDL is usually determined indirectly by measuring the capability of LDL to resist radical insult. We compared three different methods quantifying the antioxidative capacity of LDL ex vivo...

journal_title：Free radical research

authors： Malminiemi K,Palomäki A,Malminiemi O

更新日期：2000-11-01 00:00:00

abstract：:The ability of a range of dietary flavonoids to inhibit low-density lipoprotein (LDL) oxidation in vitro was tested using a number of different methods to assess oxidative damage to LDL. Overall quercetin was the most effective inhibitor of oxidative damage to LDL in vitro. On this basis, a diet enriched with onions a...

journal_title：Free radical research

authors： O'Reilly JD,Sanders TA,Wiseman H

更新日期：2000-10-01 00:00:00

abstract：:Oxidative stress appears to be implicated in the pathogenesis of various diseases including hepatotoxicity. Although intracellular Ca2+ signals have been suggested to play a role in the oxidative damage of hepatocytes, the sources and effects of oxidant-induced intracellular Ca2+ increases are currently debatable. Thu...

journal_title：Free radical research

authors： Kim JA,Kang YS,Lee SH,Lee YS

更新日期：2000-09-01 00:00:00